LOCUS       pLV-[hsa-miR-377]        9123 bp    DNA     circular      3-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7044..7904
                     /label=Amp(R)
     promoter        8105..8645
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5649..5883
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6458..6913)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5955..6085
                     /label=SV40 pA
     misc_feature    6086..6299
                     /label=SV40 ori
     misc_feature    4970..5566
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3588
                     /label=intron
     CDS             3635..4960
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3378
                     /label=hsa-mir-377 precursor
BASE COUNT     2150 a      2369 c      2491 g      2113 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg cgagcctgca 
     3121 gctggagtca gcagggaggt cccgcaggca atgccgcctt tggtgaagag gcatctcggt 
     3181 gtgttcttgc ccgtgctgat gtttgaccct tgagcagagg ttgcccttgg tgaattcgct 
     3241 ttatttatgt tgaatcacac aaaggcaact tttgtttgag tatcaaatcc tgcttgggat 
     3301 ggcttccggg acccagtggc aagctcaggg gcatctacac ccctcccgtg agcaagaatg 
     3361 gacggggtag acgtgggtgc tagctcgagc ttttggagta cgtcgtcttt aggttggggg 
     3421 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 
     3481 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 
     3541 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 
     3601 ctacccctct agagtcgagc taccggtcgc caccatggtg agcaagggcg aggaggataa 
     3661 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     3721 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     3781 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     3841 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     3901 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     3961 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     4021 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     4081 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     4141 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     4201 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     4261 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     4321 cggcatggac gagctgtaca aggacccacc ggtcgccacc atgaccgagt acaagcccac 
     4381 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
     4441 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
     4501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
     4561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
     4621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
     4681 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
     4741 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
     4801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
     4861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga 
     4921 ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt cgacaatcaa 
     4981 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     5041 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     5101 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     5161 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     5221 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     5281 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     5341 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     5401 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     5461 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     5521 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 
     5581 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     5641 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 
     5701 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     5761 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     5821 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     5881 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     5941 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     6001 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     6061 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 
     6121 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     6181 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     6241 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 
     6301 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     6361 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     6421 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     6481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     6541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     6601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     6661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     6721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     6781 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     6841 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     6901 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     6961 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     7021 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     7081 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     7141 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     7201 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     7261 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     7321 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     7381 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     7441 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     7501 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     7561 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     7621 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     7681 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     7741 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     7801 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     7861 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     7921 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     7981 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     8041 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     8101 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     8161 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     8221 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     8281 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     8341 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     8401 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     8461 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     8521 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     8581 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     8641 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     8701 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     8761 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     8821 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
     8881 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     8941 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
     9001 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
     9061 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 
     9121 tgc 
//
